Spike S1-Biotin (SARS-CoV-2): ACE2 TR-FRET Assay Kit

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Catalog #

79949

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Description

The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.

The SARS-CoV-2 Spike S1:ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 and human ACE2 in a homogeneous 96 or 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, dye-labeled acceptor and an inhibitor for one hour. Then the TR-FRET signal is measured using a fluorescence reader.

Spike S1-Biotin SARS-CoV-2 ACE2 TR-FRET Assay Kit

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Synonyms

SARS-CoV-2 inhibition kit, ACE2 inhibition kit, SARS TR-FRET kit, ACE2 homogenous kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

TR-FRET

Supplied As

96 reaction format (79949-1) or 384 reaction format (79949-2)

Materials Required But Not Supplied

Fluorescence microplate reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format

96 Reactions:

Catalog Number	Component	Amount	Storage
100705	ACE2, His-Tag, Eu-labeled*	2 µg	-80°C	Avoid multiple freeze/ thaw cycles!
100679	Spike S1, Fc fusion, Avi-tag, Biotin-Labeled (SARS-CoV-2)*	25 µg	-80°C
	Dye-labeled acceptor	2x10 µl	-20°C
79953	3x ACE2-Spike TR-FRET Buffer	4 ml	-20°C
79696	96-well white microplate	1	Room Temp

*The concentration of ACE2 and Spike S1 is lot-specific and will be indicated on the tubes containing the enzyme.

384 Reactions:

Catalog Number	Component	Amount	Storage
100705	ACE2, His-Tag, Eu-labeled*	2x2 µg	-80°C	Avoid multiple freeze/ thaw cycles!
100679	Spike S1, Fc fusion, Avi-tag, Biotin-Labeled (SARS-CoV-2)*	2x25 µg	-80°C
	Dye-labeled acceptor	3x10 µl	-20°C
79953	3x ACE2-Spike TR-FRET Buffer	4 ml	-20°C
	384-well white microplate	1	Room Temp

*The concentration of ACE2 and Spike S1 is lot-specific and will be indicated on the tubes containing the enzyme.

References

Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. Science, 4 March 2020. In Press. DOI: 10.1126/science.abb2762

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Spike S1-Biotin (SARS-CoV-2): ACE2 TR-FRET Assay Kit

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