Spike S1 RBD (B.1.617.2.1, Delta Plus Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) HiP™ Recombinant

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Catalog #

101182

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Description

Recombinant SARS-CoV-2 Spike protein, RBD (Receptor Binding Domain) encompassing amino acids 319-541. This protein corresponds to SARS-CoV-2 Variant B.1.617.2.1 also known as variant Delta Plus originally identified in India, and contains RBD mutations K417N, L452R and T478K. The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.

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Synonyms

sars cov 2 Spike, Lineage B.1.617.2.1, Delta Plus variant

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Biotin-Avidin Pulldown

Gel Filtration Trace

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Construct

Spike S1 RBD (B.1.617.2.1 Variant) (319-541-Avi-His)-(Biotin) (SARS-CoV-2)

Mutation

K417N, L452R, T478K

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

28 kDa + glycans

Amino Acids

319-541

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Aggregation

<10%

Genbank #

MN908947

UniProt #

P0DTC2

Tag(s)

C-terminal Avi-His-tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

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Spike S1 RBD (B.1.617.2.1, Delta Plus Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) HiP™ Recombinant

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