Spike S1 (B.1.1.529 BA.1, Omicron Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) Recombinant

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101334

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Description

Recombinant SARS-CoV-2 Spike protein S1 subunit, encompassing amino acids 16-685. This protein corresponds to SARS-CoV-2 Variant B.1.1.529 BA.1, also known as variant Omicron BA.1 originally identified in South Africa, and contains genetic alterations indicated below. It was constructed with a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

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Synonyms

sars cov 2 Spike, sublineage BA.1

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

ACE2-Eu: Spike S1 [Biotinylated] (Omicron Variant) Binding

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Construct

Spike S1 (B.1.1.529 BA.1 Variant) (16-685-Avi-His) - (Biotin) (SARS-CoV-2)

Mutation

A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, l212I, Ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

78 kDa + glycans

Amino Acids

16-685

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

MN908947

UniProt #

P0DTC2

Tag(s)

C-terminal Avi-His-tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay run according to the Spike S1 (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 TR-FRET Assay Kit (BPS Bioscience #78358). 5 ng of ACE2-Eu, 400-fold diluted Dye-labeled acceptor, and various amounts of Spike S1[B] incubated for 1 hour at room temperature. TR-FRET measured at ex340 ± 20nm, em620 ± 10 nm; and ex340 ± 20nm, em665 ± 10 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike S1 (B.1.1.529 BA.1, Omicron Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) Recombinant

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