Spike S1 (Wild-Type) (SARS-CoV-2): ACE2 TR-FRET Assay Kit

Catalog #

78281

$1,070 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Spike S1 (Wild-Type) (SARS-CoV-2): ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 (Wild-Type) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and the dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET).

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Synonyms

SARS-CoV-2 inhibition kit, ACE2 inhibition kit, SARS TR-FRET kit, ACE2 homogenous kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

TR-FRET

Supplied As

Homogeneous 384 reaction format

Materials Required But Not Supplied

Fluorescence microplate reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format

Catalog #	Name	Amount	Storage
100705	ACE2, His-Tag, Eu-labeled*	2 x 2 µg	-80°C
100720	Spike S1*, Fc fusion, Avi-tag, Biotin-Labeled (16-685) (SARS-CoV-2)	2 x 25 µg	-80°C
	Dye-labeled acceptor	3 x 10 µl	-20°C
79953	3x ACE2-Spike TR-FRET Buffer	4 ml	-20°C
79969	384-well white microplate	1	Room Temp.

*The initial concentration of both ACE2 and Spike S1 is lot-specific and will be indicated on the tube containing the protein.

Background

The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection.

References

1. Hoffmann, M. et al. 2020. Cell, 181:1-10
2. Yan, R. et al. 2020. Science 367(6485):1444-1448

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Instructions for Use

See assay protocol for detailed instructions

Applications

This kit is useful for screening for inhibitors of the interaction between SARS-CoV-2 Spike S1 and human ACE2.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide

Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

Keep final DMSO concentration at or below 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike S1 (Wild-Type) (SARS-CoV-2): ACE2 TR-FRET Assay Kit

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