ACE2: Spike S1 RBD (SARS-CoV-2) Inhibitor Screening Assay Kit

Catalog #
$660 *
Size: 96 reactions
*US Pricing only. For international pricing, please contact your local distributor.

This kit is based on binding to the mouse Fc region. If your sample includes human serum, it may interfere with the assay and create a false positive signal. In this instance, we suggest using the SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit, BPS Bioscience #79945.

Coronavirus disease 2019 (COVID-19) increases the risk of developing Acute Respiratory Distress Syndrome (ARDS), which is often fatal at the late stages of the infection when the SAR-CoV-2 virus causes significant damage to the lungs. As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.

ACE2 Spike S1 RBD SARS-CoV-2

The ACE2:SARS-CoV-2 Spike S1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of Fc-tagged Spike protein by HRP-labeled Anti-mouse-Fc. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a nickel-coated 96-well plate. Next, SARS-CoV-2 Spike S1 is incubated with ACE2 on the plate. Finally, the plate is treated with Anti-mouse-Fc-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.

This product has been cited 25 times.

ACEH, wild type
Product Info
Storage and Usage
Assay Kit Format
Supplied As
This kit comes in a convenient 96-well format, with purified ACE2 and SARS-CoV-2 Spike S1 proteins, HRP-labeled anti-mouse Fc region antibody, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Catalog # Component Amount Storage
100684 SARS-CoV-2 Spike S1 Protein (RBD), mFc Tag  2 μg  -80°C Avoid
thaw cycles!
11003  ACE2, His-Tag  5 μg  -80°C
79311 3x Immuno Buffer 1  50 ml  -20°C
79728 Blocking Buffer 2  50 ml  +4°C
52130H Secondary HRP-labeled antibody 1 (mouse) 10 μl  -80°C
79670 ELISA ECL substrate A (transparent bottle)  6 ml Room
ELISA ECL substrate B (brown bottle)  6 ml  Room
  Nickel-coated 96-well white microplate  1  +4°C
UniProt #
ACE2: Q9BYF1; Spike S1 RBD: P0DTC2

Hoffmann, M. et al. 2020. Cell, 181(2):271-280.e1-e5
Yan, R. et al. 2020. Science, 367(6485):1444-1448