ACE2 - CHO Recombinant Cell Line

Catalog #

79959

$4,500 *

Size: 2 vials

Qty

Bulk/Custom

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Description

Recombinant clonal stable CHO cell line constitutively expressing full length human ACE2, Genbank #NM_021804.3). Surface expression of ACE2 was confirmed by flow cytometry.

This product has been cited 1 time.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

ACE2 cell line, ACEH stable cell line

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Product Data Gallery

Expression of ACE2 validated by flow cytometry

Transduction of ACE2-CHO Cells using SARS-CoV-2 Spike Pseudotyped Lentivirus

Product Info

Storage and Usage

Citations1

Host Cell Line

CHO K1 cell line, Chinese Hamster Ovary

Supplied As

Each vial contains ~ 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS.

Materials Required But Not Supplied

• Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone
#SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).
• Growth Medium 3J (BPS Bioscience, #79974): Thaw Medium 3 (BPS Bioscience, #60186) plus 5 μg/ml of Puromycin (Takara, #631306) to ensure recombinant expression.
• Spike (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter) (BPS Bioscience, #79942)
• Bald lentiviral pseudovirion (Luciferase reporter) (BPS Bioscience, #79943)
• Anti-SARS-CoV-2 Spike antibody (clone AM001414, Active motif, #91361)
• Anti-ACE2 antibody (R&D systems, #AF933)
• Recombinant ACE2 protein (BPS Bioscience, #11003)
• 96-well tissue culture treated, white clear-bottom assay plate (Corning, #3610)
• ONE-Step™ luciferase assay system (BPS Bioscience, #60690)

Genbank #

NM_021804.3

UniProt #

Q9BYF1

Mycoplasma Testing

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination.

Background

Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found in the outer space of cells in the lungs, arteries, heart, kidney, and intestines. ACE2 serves as the entry point into cells for some coronaviruses, including the SARS-CoV-2 virus that is responsible for the COVID-19 pandemic.

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience, #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3J (BPS Bioscience, #79974): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 5 μg/ml of Puromycin to ensure recombinant expression.

Cells should be grown at 37˚C with 5% CO2 using Growth Medium 3J.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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ACE2 - CHO Recombinant Cell Line

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