ACE2, Fc Fusion (lgG1), Avi-Tag, Biotin-Labeled HiP™ Recombinant

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Catalog #

101200

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Description

Recombinant human ACE2 (angiotensin converting enzyme 2), encompassing amino acids 18-740. The protein is fused at the C-terminus with the Fc fragment of a human IgG1 followed by a C-terminal Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.

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Synonyms

ACE2, ACEH, angiotensin I converting enzyme 2, sars, sars protease

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

ACE2 [Biotinylated] Activity

Biotin-Avidin Pulldown

Gel Filtration Trace

Product Info

Storage and Usage

Citations

Species

Human

Construct

ACE2 (18-740-Fc(lgG1)-Avi)-(Biotin)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

112 kDa + glycans

Amino Acids

18-740

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Aggregation

<10%

Genbank #

NM_021804

UniProt #

Q9BYF1

Tag(s)

C-terminal Fc-Avi-Tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to the ACE2 Inhibitor Screening Kit (BPS Bioscience #79923) with various amounts of ACE2.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Protease, Coronavirus, COVID-19

Regulatory Status

Research Use Only

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ACE2, Fc Fusion (lgG1), Avi-Tag, Biotin-Labeled HiP™ Recombinant

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