Kinase Screening & Profiling

Kinases use ATP to catalyze the transfer of a phosphate group to another molecule, generating ADP in the process. There are over 500 kinases encoded by the human genome controlling approximately 50% of all cell functions. Kinases are divided into distinct families based upon their substrates: lipid, nucleotide, carbohydrate, and protein.

Many kinases act as proto-oncogenes which, when overexpressed or mutated, support oncogenic transformation and tumorigenicity. Therefore, kinases represent attractive therapeutic targets in oncology.

Kinase Assay

A purified active kinase is incubated with a test inhibitor. The reaction is initiated by adding the appropriate substrate and ATP. A luminescent reagent such as Kinase-Glo® is added for detection of the reaction products.