Kinase Screening & Profiling
Kinases use ATP to catalyze the transfer of a phosphate group to another molecule, generating ADP in the process. There are over 500 kinases encoded by the human genome controlling approximately 50% of all cell functions. Kinases are divided into distinct families based upon their substrates: lipid, nucleotide, carbohydrate, and protein.
Many kinases act as proto-oncogenes which, when overexpressed or mutated, support oncogenic transformation and tumorigenicity. Therefore, kinases represent attractive therapeutic targets in oncology.
Our team of experts, using our in-house developed biochemical assay kits, will:
- Screen for kinase inhibitors from large compound libraries
- Compare potency with IC50 screens
- Answer your questions and guide your project in a timely manner
- Deliver detailed results promptly and on time
If follow-up studies are required, we can provide the same active proteins as the ones used in our assays.
Learn more about the process and deliverables.
A purified active kinase is incubated with a test inhibitor. The reaction is initiated by adding the appropriate substrate and ATP. A luminescent reagent such as Kinase-Glo® is added for detection of the reaction products.
JH2 Domain Inhibitor Screening Assay
Fluorescence Polarization Assay: The assay uses a fluorescent JH2-binding probe that emits differently polarized light depending on its binding with a JH2 domain. Addition of a binding inhibitor decreases the fluorescence polarization signal in a dose dependent manner. This is a homogeneous assay.
Available Service Assays
Note: Not all targets will have a commercially available reference control. Please inquire with our team if you need to furnish your own controls for specific targets.
- CRISPR Services
- CRISPR/Cas9 Kinase Knockout Lentivirus Panel
- PROTAC® Optimization Services
- Custom Biochemical Assay Development
- Assay Kits
- Cell Lines
- Lipid Kinases
- Tyrosine Kinases
- Serine-Threonine Kinases
- CRISPR/Cas9 Kinase Knockout Lentivirus Library
- Kinase (Human) CRISPR/Cas9 Lentivirus (Integrating)