Cell-Based Assays and Expression Kits

Cells can be engineered to serve as readouts for cell-based assays as a complement or alternative to biochemical assays. A variety of cell-based assay types can be used for research and development applications, including signaling pathway analysis, inhibitor/activator studies, CAR-T cell functional screening, ADCC assays, high-throughput screening systems and much more. We provide a variety of cell-based assay expression kits to allow you to engineer your own cells for research in your desired model system or cell library.

For co-culture cellular assays to identify checkpoint molecule inhibitors, we provide a number of kits with luciferase reporter effector cells. The kits include expression vectors for TCR activator and PD-L1 or PD-L2, growth-arrested PD-1/NFAT reporter Jurkat cells, cell assay medium, ONE-Step™ Luciferase Assay reagent, and an anti-PD-1 neutralizing antibody as a positive control. These kits enable screening of inhibitors or activators of PD-1 signaling in a cellular context or characterize the biological activity of PD-1 and its interactions with ligands.