Protease Screening & Profiling

Proteases are catalytic enzymes found in all forms of life, including viruses, that regulate a wide range of vital physiological processes. In animals, they are critical for digestion of food, lysosome and proteasome degradation, apoptosis, blood coagulation, cell cycle regulation, cell signaling, and more. The dysregulation of protease activity can lead to pathologies such as arthritis and inflammatory diseases, cardiovascular disorders, cancer, osteoporosis, Alzheimer’s, and other neurological disorders. Several small molecule protease inhibitors are already on the market and many more are in development.

Viral proteases, such as SARS-CoV-2 3CL Pro, are essential to viral infection and replication and are attractive targets for anti-viral therapeutics.

We provide a selection of protease screening services across the main catalytic types: aspartic, cysteine, serine, and metallo- proteases. Our team of experts, using our in-house developed biochemical assay kits, can provide:

  • Screening for activity and selectivity across our full panel or just select targets
  • Determination of IC50
  • Project guidance and questions answered in a timely manner
  • Delivery of detailed results promptly and on time
SARS-CoV-2 3CL Protease Dimer

Molecular model of SARS-CoV-2 3CL protease dimer (brown/green) in complex with inhibitor GC376 (yellow) based on PDB ID 7CBT.

Inhibition curve of SARS-CoV-2 3CL protease activity

Inhibition curve of SARS-CoV-2 3CL protease activity by GC376.

If follow-up studies are required, we can provide the same active proteins as the ones used in our assays.

Learn more about the process and deliverables.

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*Alternative assay formats may be available. Inquire with our team to learn more. Export results View all selections

Note: Not all targets will have a commercially available reference control. Please inquire with our team if you need to furnish your own controls for specific targets.