CRISPR/Cas9 Kinase Knockout Lentivirus Library (Array Format)

Catalog #
78487
$7,700 *
Size: 200 µl x 649
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The CRISPR/Cas9 Kinase Knockout Lentivirus Library (Array Format) targets 619 human kinases and pseudo-kinases. A table containing the complete list of kinases and pseudo-kinases can be downloaded as an excel file from our website. The Array consists of a series of vials, with each vial containing a mixture of integrating CRISPR/Cas9 lentiviral particles targeting 5 sgRNAs for a specific gene (1 vial per gene, 5 sgRNAs per gene). The Array also includes a total of 150 control sgRNAs that do not target any gene (combined into 30 vials containing 5 control sgRNAs per vial). Thus, the Array contains a total of 649 vials and 3,245 sgRNAs. Download the table to view all kinases included. 

CRISPR Cas9 Kinase Knockout Lentivirus

The lentiviruses are replication incompetent, VSV-G pseudotyped lentiviral particles ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The SIN (self-inactivation) lentiviral backbone contains the Cas9 gene (Streptococcus pyogenes CRISPR associated protein 9) driven by an EF1A promoter, an sgRNA driven by a U6 promoter, and a puromycin selection marker.

CRISPR Cas9 Kinase Knockout Lentivirus

Schematic of the lentivector used to generate each kinase CRISPR/Cas9 Lentivirus.

The lentiviruses integrate randomly into the cellular genome to express both Cas9 and the sgRNAs. Because the lentiviruses contain Cas9, they can be used in any target cell regardless of whether the cells already express Cas9. Puromycin selection ensures high expression of both Cas9 and the sgRNAs. Knockout efficiencies will depend on the cell type and the gene of interest. Stable CRISPR/Cas9 knockout cell lines can also be generated following limiting dilution.

The library is delivered with a User Manual booklet.

Product Info
Storage and Usage
Citations
Supplied As
Each of the 649 vials come with 200 µl of lentivirus at a titer ≥1 x 107 TU/ml. The titer varies slightly with each lot; the exact titer is provided with each shipment. Each lentivirus was individually titered to ensure representation across the entire library. Cell titers were determined by measuring the lentiviral structural protein p24 (using Lenti-X GoStix Plus by Takara).
Formulation

The lentivirus particles are produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.