Fluoro-Verse™ ACE2 Inhibitor Assay Kit
The Fluoro-Verse™ Angiotensin Converting Enzyme 2 (ACE2) Inhibitor Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the exopeptidase activity of ACE2 for screening and profiling applications. The ACE2 assay kit contains enough purified ACE2 (BPS Bioscience #11003, amino acids 18-740), its substrate, and ACE2 buffer for 100 reactions.
Figure 1: Illustration of the principle behind the ACE2 fluorogenic assay.
The assay uses an ACE2 substrate in which the fluorescence emitted by Mca (7-Methoxycoumarin-4-acetic acid) is quenched by the proximal Dnp (2,4-dinitrophenyl) quencher. ACE2 is a carboxypeptidase that cleaves the C-terminus peptide bond. Thus, Mca is released from quenching upon proteolysis and emits fluorescence with excitation/emission maxima of 320/380 nm. The increase in fluorescence is proportional to ACE2 activity.
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| Catalog # | Name | Amount | Storage |
| 11003 | ACE2, His-Tag* | >1 µg | -80°C |
| ACE2 Fluorogenic Substrate | 5 µl | -80°C | |
| ACE2 Buffer | 3 ml | -20°C | |
| 79685 | 96-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
Angiotensin Converting Enzyme 2 (ACE2) is an exopeptidase that catalyzes the conversion of angiotensin II to angiotensin 1-7 and L-phenylalanine. Angiotensin II is part of the classical renin angiotensin system (RAS), a hormone system that regulates fluid balance, blood pressure and maintains vascular tone. ACE2 plays a role in lowering blood pressure and in cardiovascular health and therefore, is an attractive therapeutic target. ACE2 is also the receptor for the human respiratory coronaviruses NL63, SARS (SARS-CoV) and 2019-nCoV/SARS-CoV-2.
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