Fluoro-Verse™ ACE2 Inhibitor Assay Kit

Catalog #
$545 *
Size: 96 reactions
*US Pricing only. For international pricing, please contact your local distributor.

The Fluoro-Verse™ Angiotensin Converting Enzyme 2 (ACE2) Inhibitor Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the exopeptidase activity of ACE2 for screening and profiling applications. The ACE2 assay kit contains enough purified ACE2 (BPS Bioscience #11003, amino acids 18-740), its substrate, and ACE2 buffer for 100 reactions.

Figure 1: Illustration of the principle behind the ACE2 fluorogenic assay.
The assay uses an ACE2 substrate in which the fluorescence emitted by Mca (7-Methoxycoumarin-4-acetic acid) is quenched by the proximal Dnp (2,4-dinitrophenyl) quencher. ACE2 is a carboxypeptidase that cleaves the C-terminus peptide bond. Thus, Mca is released from quenching upon proteolysis and emits fluorescence with excitation/emission maxima of 320/380 nm. The increase in fluorescence is proportional to ACE2 activity.

Need us to run inhibitor screens or profile your compounds against ACE2? Check out our Protease Screening Services or Coronavirus Services.

ACE2, ACEH, angiotensin I converting enzyme 2, sars, sars protease
Product Info
Storage and Usage
Assay Kit Format
Catalog # Name Amount Storage
11003 ACE2, His-Tag* >1 µg -80°C
  ACE2 Fluorogenic Substrate 5 µl -80°C
  ACE2 Buffer 3 ml -20°C
79685 96-well black microplate 1 Room Temp

* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.


Angiotensin Converting Enzyme 2 (ACE2) is an exopeptidase that catalyzes the conversion of angiotensin II to angiotensin 1-7 and L-phenylalanine. Angiotensin II is part of the classical renin angiotensin system (RAS), a hormone system that regulates fluid balance, blood pressure and maintains vascular tone. ACE2 plays a role in lowering blood pressure and in cardiovascular health and therefore, is an attractive therapeutic target. ACE2 is also the receptor for the human respiratory coronaviruses NL63, SARS (SARS-CoV) and 2019-nCoV/SARS-CoV-2.