ACE2: Spike S1-Biotin (SARS-CoV-2 ) Inhibitor Screening Assay Kit

Catalog #
79945
$865 *
Size: 96 reactions
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Description

Coronavirus disease 2019 (COVID-19) increases the risk of developing Acute Respiratory Distress Syndrome (ARDS), which is often fatal at the late stages of the infection when the SAR-CoV-2 virus causes significant damage to the lungs. As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike S1 protein recognizes and attaches to the Angiotensin Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike S1 protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.

The ACE2:SARS-CoV-2 Spike S1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of Spike S1-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a nickel-coated 96-well plate. Next, SARS-CoV-2 Spike S1-Biotin is incubated with ACE2 on the plate. Finally, the plate is treated with Streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.

ACE2: Spike S1-Biotin (SARS-CoV-2 ) Inhibitor Screening Assay Kit

This product has been cited 35 times.

Synonyms
nCoV-2019, nCoV2019, ACEH Screening kit, SARS spike ELISA, ACEH ELISA
Product Info
Storage and Usage
Citations35
Assay Kit Format
Chemiluminescent
Species
SARS-CoV-2
Supplied As
This kit comes in a convenient 96-well format, with purified ACE2 and SARS-CoV-2 Spike S1 proteins, Streptavidin-HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format
Catalog # Component Amount Storage
100679 SARS-CoV-2 Spike S1, Fc Fusion, Avi-tag, Biotin-labeled* 10 µg -80°C Avoid
multiple
freeze/
thaw
cycles
11003 ACE2, His-Tag* 5 µg -80°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C
79742 Streptavidin-HRP 10 µl -20°C

79670
ELISA ECL substrate A (transparent bottle) 6 ml +4°C
ELISA ECL substrate B
(brown bottle)
6 ml +4°C
  Nickel-coated 96-well white microplate 1 +4°C

*The concentrations of Spike S1 and ACE2 are lot-specific and will be indicated on the tubes containing the protein.

UniProt #
ACE2: Q9BYF1; Spike S1: P0DTC2 SPIKE_WCPV
References

Hoffmann, M. et al. 2020. Cell 181:1-10
Yan, R. et al. 2020. Science 367(6485): 1444-1448.