ONE-Step™ Luciferase Assay System
The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary reagents for cell lysis and luciferase quantitation.
- Sensitive: highly sensitive detection of firefly luciferase activity.
- Stable: the signal is stable for more than two hours, providing flexibility regarding incubation time.
- Convenient: simple one-step, homogeneous protocol.
- High-throughput: the one-step protocol minimizes handling steps to support high-throughput screening applications.
- Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.
- Instrumentation: does not require a luminometer with injectors.
This product has been cited 94 times.
- Clear-bottom, white multiwell tissue culture plates that are compatible with the luminometer being used
- Mammalian cells that express firefly luciferase
- Appropriate cell culture medium
- Laboratory platform shaker
- Luminometer
10 ml Size
| Catalog # | Component | Amount | Storage |
| 78262-1 | Luciferase Reagent Buffer (Component A) | 10 ml | -20°C |
| 78263-1 | Luciferase Reagent Substrate (Component B) | 100 µl | -20°C Protect from light. |
Each kit contains sufficient reagents to perform 100 assays of 100 µl each in a 96-well plate.
100 ml Size
| Catalog # | Component | Amount | Storage |
| 78262-1 | Luciferase Reagent Buffer (Component A) | 2 x 50 ml | -20°C |
| 78263-1 | Luciferase Reagent Substrate (Component B) | 2 x 500 µl | -20°C Protect from light. |
Each kit contains sufficient reagents to perform 1000 assays of 100 µl each in 96-well plates.
500 ml Size
| Catalog # | Component | Amount | Storage |
| 78262-1 | Luciferase Reagent Buffer (Component A) | 5 x 100 ml | -20°C |
| 78263-1 | Luciferase Reagent Substrate (Component B) | 5 x 1 ml | -20°C Protect from light. |
Each kit contains sufficient reagents to perform 5000 assays of 100 µl each in 96-well plates.
1 L Size
| Catalog # | Component | Amount | Storage |
| 78262-1 | Luciferase Reagent Buffer (Component A) | 10 x 100 ml | -20°C |
| 78263-1 | Luciferase Reagent Substrate (Component B) | 10 x 1 ml | -20°C Protect from light. |
Each kit contains sufficient reagents to perform 10,000 assays of 100 µl each in 96-well plates.
Luciferase is the general term given to a class of oxidative enzymes which catalyze reactions that give off light, a process known as bioluminescence (Figure 1).
Enzymatic activity is directly proportional to the expression of luciferase in the cells and can be quantified upon addition of the appropriate substrate.
Figure 1. Bioluminescent reaction catalyzed by luciferase.
In the luciferase reaction, the cells are lysed with luciferase substrate containing D-luciferin. Firefly luciferase expressed in reporter cells catalyzes the conversion of D-luciferin to oxyluciferin using ATP as a co-substrate, and in the presence of Mg2+, in a reaction that gives off light. The amount of light is proportional to the amount of luciferase present in the reaction. Luminescence is read using a luminometer.
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