Bald Lentiviral Pseudovirion (Luciferase Reporter)

Catalog #

79943

$875 *

Size: 500 µl x 2

Qty

Bulk/Custom

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Description

The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains the firefly luciferase gene driven by a CMV promoter (Figure 1) as the reporter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

Figure 1. Schematic of the Luciferase Reporter in Bald Lentiviral Pseudovirion (Luciferase Reporter)

Figure 1. Schematic of the Luciferase Reporter in Bald Lentiviral Pseudovirion (Luciferase Reporter)

This product has been cited 17 times.

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Synonyms

Luciferase reporter SARS-Cov-2, glycoprotein reporter, viral entry reporter

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Product Data Gallery

Product Info

Storage and Usage

Citations17

Supplied As

Since the virus is lacking the envelope glycoproteins and cannot transduce target cells, functional titer of this product cannot be determined. Based on p24 values, the approximate lentiviral particles (LP) of this product is ~ 10⁹ LP/ml.

Materials Required But Not Supplied

• HEK293 growth medium or use Thaw Medium 9 (BPS Bioscience #79665): MEM with 10% FBS, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate
• HEK293 cells (ATCC#CRL-1573)
• ACE2-HEK293 Recombinant Cell Line (BPS Bioscience, #79951)
• SARS-CoV-2 Spike Pseudotyped lentivirus (Luciferase reporter) (BPS Bioscience, #79942)
• Polybrene (Millipore, # TR-1003-G)
• 96-well tissue culture treated, white clear-bottom assay plate (Corning, #3610)
• ONE-Step™ luciferase assay system (BPS Bioscience, #60690)

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Biosafety

None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Bald Lentiviral Pseudovirion (Luciferase Reporter)

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