Spike S1 RBD, Mouse Fc-fusion (SARS-CoV-2) Recombinant

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Catalog #

100684

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Description

Recombinant SARS-CoV-2 Spike protein, RBD (Receptor Binding Domain) encompassing amino acids 319-541. The protein is fused in C-terminal with the Fc fragment of a mouse IgG1. The recombinant protein was affinity purified.

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Synonyms

nCoV spike S1, Spike Glycoprotein S1, novel coronavirus spike S1

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

ACE2: SARS-CoV-2 Spike Interaction

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Host Species/Expression System

HEK293

Purity

≥90%

Supplied As

Aqueous buffer solution.

Format

Aqueous buffer solution

Formulation

8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

50 kDa

Amino Acids

319-541

Genbank #

QHD43416.1

UniProt #

P0DTC2

Tag(s)

C-terminal mFc-tag

References

1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10.
2. Walls, A. C. et al., Cell. 2020 Mar; 180(2):281-292.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to the ACE2: Spike RBD (SARS-CoV-2) Inhibitor Screening Assay Kit (BPS Bioscience, #79936) with SARS-CoV-2 Spike titrated from 80 ng- 0 ng.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Notes

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike S1 RBD, Mouse Fc-fusion (SARS-CoV-2) Recombinant

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