Spike S1 (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 TR-FRET Assay Kit
The Spike S1 (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 (B.1.1.529 BA.1, Omicron Variant) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and the dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET).
| Catalog # | Name | Amount | Storage |
| 100705 | ACE2, His-Tag, Eu-labeled* | 2 x 2 µg | -80°C |
| 101334 | Spike S1 (B.1.1.529 BA.1, Omicron Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2)* | 2 x 25 µg | -80°C |
| Dye-labeled acceptor | 3 x 10 µl | -20°C | |
| 79953 | 3x ACE2-Spike TR-FRET Buffer | 4 ml | -20°C |
| 79969 | 384-well white microplate | 1 | Room Temp |
*The initial concentrations of ACE2 and Spike S1 are lot-specific and will be indicated on the tubes containing the proteins.
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. SARS-CoV-2 Variant B.1.1.529 BA.1, also known as Omicron variant, was originally discovered in South Africa and has recently become a global variant of concern. This variant displays increased transmissibility and infectivity compared to previous known variants.
Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection. The SARS-CoV-2 Spike (B.1.1.529 BA.1, Omicron Variant) protein in this kit consists of the S1 protein, which includes the ACE2 binding site. Compared to the wild-type strain, it contains mutations A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, Ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, K484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H.
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