Spike S1 (13-665), Fc Fusion, Avi-tag (SARS-CoV-2) Recombinant

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Catalog #

100678

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Description

Recombinant SARS-CoV-2 Spike protein S1 subunit, encompassing amino acids 13-665. The protein is fused in C-terminal with the Fc fragment of human IgG followed by an Avi-Tag™. The recombinant protein was affinity purified.

This product has been cited 1 time.

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Synonyms

SARS-CoV s1, coronavirus spike S1

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Spike S1 Fc: ACE2 [Biotinylated] Binding

Product Info

Storage and Usage

Citations1

Species

SARS-CoV-2

Construct

Spike S1 (13-665)-Fc(IgG1)-Avi) (SARS-CoV-2)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

102 kDa + glycans

Amino Acids

13-665

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

MN908947

UniProt #

P0DTC2 SPIKE_WCPV

Tag(s)

C-terminal Avi-tag

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was done according to the Spike S1 (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit (BPS Bioscience #79954). 100 ng of Spike S1 #100678 was coated overnight. Various amounts of ACE2 [Biotinylated] (BPS Bioscience #100665) added and incubated for 1 hour, followed by 1 hour incubation with streptavidin-HRP, and addition of colorimetric HRP substrate and 1 N HCl. Absorbance measure at 450 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike S1 (13-665), Fc Fusion, Avi-tag (SARS-CoV-2) Recombinant

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