Spike S1 (P.1; Gamma Variant) (SARS-CoV-2): ACE2 TR-FRET Assay Kit
The Spike S1 (P.1; Gamma Variant) (SARS-CoV-2): ACE2 TR-FRET Assay Kit is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 (P.1; Gamma Variant) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET).
Fluorescence microplate reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
| Catalog # | Name | Amount | Storage |
| 100705 | ACE2, His-Tag, Eu-labeled* | 2 x 2 µg | -80°C |
| 101080 | Spike S1* (P.1), Avi-His-tag, Biotin Labeled (16-685) (SARS-CoV-2) | 2 x 25 µg | -80°C |
| Dye-labeled acceptor | 3 x 10 µl | -20°C | |
| 79953 | 3x ACE2-Spike TR-FRET Buffer | 4 ml | -20°C |
| 79969 | 384-well white microplate | 1 | Room Temp |
*The initial concentration of both ACE2 and Spike S1 is lot-specific and will be indicated on the tube containing the protein.
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein binds to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection.
The SARS-CoV-2 variant P.1, also known as Gamma variant, was first identified in Brazil in the summer of 2020. This variant has many mutations in the Spike protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y) that may lead to higher transmissibility and infectivity.
1. Hoffmann, M. et al. Cell, 181:1-10
2. Yan, R. et al. 2020. Science 367(6485):1444-1448
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