Recombinant human ACE2 (Angiotensin converting enzyme 2), encompassing amino acids 18-740. This construct contains a C-terminal His-tag (10xHis). The protein was affinity purified and labeled with trFluor™ Eu.
8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
MW
85 kDa + glycans
Amino Acids
18-740
Glycosylation
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Genbank #
NM_021804
UniProt #
Q9BYF1
Tag(s)
C-terminal His-tag
Label
trFluor™ Eu (Ex/Em = 346nm / 617 nm) is a fluorophore with large Stokes shifts and an extremely long emission half-live. It is ideal for applications that require high sensitivity and for time-resolved fluorometry because a delay between excitation and the detection of emission minimizes interference. EU-labeled probes are characterized by a relatively high stability and are often insensitive to quenching. trFluor™ Eu is covalently and randomly conjugated to the target protein.
References
1. Keider, S., et al., Nephrology Dialysis Transplantation. 2007 22(2): 597-601. 2. Donoghue, M., et al., Circulation Research. 2002 Sep;87:e1-e9
Storage/Stability
At least 6 months at -80°C.
Assay Conditions
Assay was performed according to the Spike S1-Biotin (SARS-CoV-2): ACE2 TR-FRET Assay Kit (BPS Bioscience #79949-1) with Spike S1-Biotin (BPS Bioscience #100679) enzyme titrated from 200 ng/rxn – 16 ng/rxn and ACE-Eu at 12.5 ng/rxn.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.