Spike S1 (K417T, E484K, N501Y), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) Recombinant

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Catalog #

101082

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Description

Recombinant SARS-CoV-2 Spike protein S1 subunit, encompassing amino acids 16-685. This protein contains three mutations of interest K417T, E484K and N501Y (found in the RBD domain of some variants of concern). The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Construct

Spike S1 (K417T, E484K, N501Y) (16-685-Avi-His)-(Biotin) (SARS-CoV-2)

Mutation

K417T, E484K, N501Y

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

78 kDa + glycans

Amino Acids

16-685

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

MN908947

UniProt #

P0DTC2

Tag(s)

C-terminal Avi-His-tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

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Spike S1 (K417T, E484K, N501Y), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) Recombinant

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