Spike S1 (K417T, E484K, N501Y) (SARS-CoV-2): ACE2 TR-FRET Assay Kit
The Spike S1 (K417T, E484K, N501Y) (SARS-CoV-2): ACE2 TR-FRET Assay is designed to measure the inhibition of the binding between SARS-CoV-2 Spike S1 (K417T, E484K, N501Y) and human ACE2 in a homogeneous 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; the test inhibitor compound is incubated with biotinylated Spike S1, Eu-labeled ACE2, and dye-labeled acceptor for one hour. Then the TR-FRET signal is measured using a fluorescence reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET).
Fluorescence microplate reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
| Catalog # | Name | Amount | Storage |
| 100705 | ACE2, His-Tag, Eu-labeled* | 2 x 2 µg | -80°C |
| 101082 | Spike S1 (K417T, E484K, N501Y), Avi-His-tag, Biotin Labeled (16-685) (SARS-CoV-2)* | 25 µg | -80°C |
| Dye-labeled acceptor | 3 x 10 µl | -20°C | |
| 79953 | 3x ACE2-Spike TR-FRET Buffer | 4 ml | -20°C |
| 79969 | 384-well white microplate | 1 | Room Temp |
*The initial concentration of both ACE2 and Spike S1 is lot-specific and will be indicated on the tube containing the protein.
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein binds to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and human ACE2 may offer some protection against the viral infection.
This SARS-CoV-2 Spike S1 mutant contains three critical mutations K417T, E484K, and N501Y found in the P.1 variant (Gamma variant) originally discovered in Brazil.
1. Hoffmann, M. et al. Cell, 181:1-10
2. Yan, R. et al. Science 367(6485):1444-1448
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