Spike S1 (16-685), Fc Fusion, Avi-tag, Biotin-labeled (SARS-CoV-2) Recombinant

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Catalog #

100720

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Description

Recombinant SARS-CoV-2 Spike protein S1 subunit, encompassing amino acids 16-685. The protein is fused in C-terminal with the Fc fragment of human IgG1 followed by an Avi-Tag™. The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

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Synonyms

SARS-CoV s1, coronavirus spike S1

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

ACE2: SARS-CoV-2 Inhibition

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Construct

Spike S1 (16-685-Fc (lgG1)-Avi)-(Biotin) (SARS-CoV-2)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

104 kDa + glycans

Amino Acids

16-685

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

MN908947

UniProt #

P0DTC2 SPIKE_WCPV

Tag(s)

C-terminal Fc-Avi-tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

SARS-CoV-2 S1(BPS Bioscience #100720) and ACE2 binding assay was done using BPS ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit (BPS Bioscience #79936). SARS-CoV-2 S1 (BPS Bioscience #100720) protein and Streptavidin-HRP were used instead of SARS-CoV-2 Spike protein (RBD) and anti-mouse Fc-HRP respectively.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike S1 (16-685), Fc Fusion, Avi-tag, Biotin-labeled (SARS-CoV-2) Recombinant

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