Spike S1 RBD (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit

Catalog #
78018
$765 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Receptor Binding Domain (RBD) of Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.


The Spike RBD S1 (SARS-CoV-2):ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike RBD protein is attached to a 96-well transparent plate. Next, ACE2-Biotin is incubated with Spike RBD on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

 

This product has been cited 2 times.

Synonyms
SARS-CoV-2 inhibition kit, ACE2 inhibition kit, SARS Colorimetric kit, Spike RBD kit
Product Info
Storage and Usage
Citations2
Assay Kit Format
Colorimetric
Species
SARS-CoV-2
Supplied As
This kit comes in a convenient 96-well format, with purified SARS-CoV-2 Spike S1 RBD and ACE2-Biotin proteins, streptavidin-HRP, colorimetric HRP substrate, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used, but the sensitivity of the
assay will be greatly reduced.

Format
Catalog # Component Amount Storage
100696 Spike S1 RBD, Avi-His-tag (SARS-CoV-2) 5 µg -80°C Avoid
multiple
freeze/
thaw
cycles!
100665 ACE2, His-Avi-Tag, Biotin-labeled HiP™ 5 µg -80°C
79742 Streptavidin-HRP 10 µl +4°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C
79651 Colorimetric HRP substrate 10 ml +4°C
79964 Transparent 96-well white microplate 1 Room
Temp
 
UniProt #
Spike S1 RBD: P0DTC2 SPIKE_WCPV; ACE2: Q9BYF1
References

Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. 2020. Science, 367(6485):1444-1448.