Spike S1 RBD (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Receptor Binding Domain (RBD) of Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.
The Spike RBD S1 (SARS-CoV-2):ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike RBD protein is attached to a 96-well transparent plate. Next, ACE2-Biotin is incubated with Spike RBD on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
This product has been cited 2 times.
PBS (Phosphate buffered saline)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used, but the sensitivity of the
assay will be greatly reduced.
| Catalog # | Component | Amount | Storage | |
| 100696 | Spike S1 RBD, Avi-His-tag (SARS-CoV-2) | 5 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 100665 | ACE2, His-Avi-Tag, Biotin-labeled HiP™ | 5 µg | -80°C | |
| 79742 | Streptavidin-HRP | 10 µl | +4°C | |
| 79311 | 3x Immuno Buffer 1 | 50 ml | -20°C | |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C | |
| 79651 | Colorimetric HRP substrate | 10 ml | +4°C | |
| 79964 | Transparent 96-well white microplate | 1 | Room Temp |
|
Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. 2020. Science, 367(6485):1444-1448.
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