Spike S1 RBD (B.1.1.7; Alpha Variant), Avi-His-Tag, Biotin-Labeled (SARS-CoV-2) HiP™ Recombinant
Recombinant SARS-CoV-2 Spike protein, RBD (Receptor Binding Domain) encompassing amino acids 319-541. This protein corresponds to SARS-CoV-2 Variant B.1.1.7, also known as variant Alpha originally identified in the United Kingdom, and contains mutation N501Y. It was constructed with a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
≥90%
Aqueous buffer solution
8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.
For more information on enzymatic biotinylation, please see our Tech Note.