Spike S1 RBD (SARS-CoV-2): ACE2 Inhibitor Screening Assay Kit

Catalog #
$675 *
Size: 96 reactions
*US Pricing only. For international pricing, please contact your local distributor.

The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.

The Spike S1 RBD (SARS-CoV-2): ACE2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of bound His-labeled ACE2 by HRP-labeled Anti-His. Only a few simple steps on a microtiter plate are required for the assay. First, SARS-CoV-2 Spike S1 is coated on a 96-well plate. Next, ACE2-His is incubated with SARS-CoV-2 Spike S1 on the plate. Finally, the plate is treated with Anti-His-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.

Spike S1 RBD Coronavirus

This product has been cited 15 times.

ACEH, RBD inhibitor screening, SARS CoV 2 inhibition assay
Product Info
Storage and Usage
Assay Kit Format
Supplied As
This kit comes in a convenient 96-well format, with His-labeled ACE2, purified SARS-CoV-2 Spike S1 protein, HRP-labeled anti-His antibody, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Catalog # Component Amount Storage
100684 SARS-CoV-2 Spike S1 Protein (RBD), mFc Tag 5 μg -80°C Avoid
11003 ACE2, His-Tag 5 μg -80°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C
  Anti-His-HRP 10 μl -80°C
79670 ELISA ECL substrate A (transparent bottle) 6 ml Room
ELISA ECL substrate B (brown bottle) 6 ml Room
79699 96-well white microplate 1 +4°C

Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. Science, In Press 4 March 2020. DOI: 10.1126/science.abb2762