Spike S1 (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection
The SARS-CoV-2 Spike S1:ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike S1 protein is attached to a 96-well transparent plate. Next, ACE2-Biotin is incubated with Spike S1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
This product has been cited 11 times.
PBS (Phosphate buffered saline)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used, but sensitivity of the assay
will be greatly reduced.
| Catalog # | Component | Amount | Storage | |
| 100678 | Spike S1, Fc Fusion, Avi-tag (SARS-CoV-2) | 10 µg | -80°C | Avoid multiple freeze/ thaw cycles |
| 100665 | ACE2, His-Avi-Tag, Biotin-labeled HiP™ | 2 µg | -80°C | |
| 79742 | Streptavidin-HRP | 10 µl | +4°C | |
| 79311 | 3x Immuno Buffer 1 | 50 ml | -20°C | |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C | |
| 79651 | Colorimetric HRP substrate | 10 ml | +4°C | |
| 79964 | Transparent 96-well microplate | 1 | Room Temp |
|
*The concentration of Spike S1 and ACE2 is lot-specific and will be indicated on the tubes containing the proteins
Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. 2020. Science, 367(6485):1444-1448.
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