Spike S1 (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit

Catalog #
79954
$845 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection

The SARS-CoV-2 Spike S1:ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of ACE2-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, Spike S1 protein is attached to a 96-well transparent plate. Next, ACE2-Biotin is incubated with Spike S1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

79954

This product has been cited 17 times.

Synonyms
SARS-CoV-2 inhibition kit, ACE2 inhibition kit, SARS TR-FRET kit, ACE2 homogenous kit
Product Info
Storage and Usage
Citations17
Assay Kit Format
Colorimetric
Species
SARS-CoV-2
Supplied As
This kit comes in a convenient 96-well format, with purified SARS-CoV-2 Spike S1 and ACE2-Biotin proteins, streptavidin-HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used, but sensitivity of the assay
will be greatly reduced.

Format
Catalog # Component Amount Storage
100678 Spike S1, Fc Fusion, Avi-tag (SARS-CoV-2) 10 µg -80°C Avoid
multiple
freeze/
thaw
cycles
100665 ACE2, His-Avi-Tag, Biotin-labeled HiP™ 2 µg -80°C
79742 Streptavidin-HRP 10 µl +4°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C
79651 Colorimetric HRP substrate 10 ml +4°C
79964 Transparent 96-well microplate 1 Room
Temp

*The concentration of Spike S1 and ACE2 is lot-specific and will be indicated on the tubes containing the proteins

UniProt #
Spike S1: P0DTC2 SPIKE_WCPV; ACE2: Q9BYF1
References

Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. 2020. Science, 367(6485):1444-1448.