Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant), His-Tag (SARS-CoV-2) Recombinant

Catalog #

101343

$330 *

Size: 100 µg

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Description

Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein corresponds to SARS-CoV-2 Variant B.1.1.529 BA.1 also known as variant Omicron originally identified in South Africa, and contains Omicron Spike mutations listed below. The construct also contains mutations 682RRAR685>A, K986P and V987P, and a T4 trimerization domain followed by a His-tag (6xHis) in C-terminal. The recombinant protein was affinity purified.

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Synonyms

sars cov 2 Spike, sublineage BA.1

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) Activity

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Construct

Spike Trimer (S1+S2) (B.1.1.529 BA.1 Variant) (16-1213-His) (SARS-CoV-2)

Mutation

A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, Ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, K484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F

Host Species/Expression System

HEK293

Purity

80%

Format

Aqueous buffer solution

Formulation

8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

137 kDa + glycans

Amino Acids

16-1213

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

MN908947

UniProt #

P0DTC2

Tag(s)

C-terminal His-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to the Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit (BPS Bioscience #78365) with serial dilutions of ACE2, His-Avi-Tag, Biotinlabeled HiP™ (BPS Bioscience #100665). The dilutions ranged from 0 to 1 µM in 3-fold dilutions.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant), His-Tag (SARS-CoV-2) Recombinant

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