Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit
The Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between the SARS-CoV-2 Omicron variant Spike Trimer and human ACE2. The SARS-CoV-2 Spike Trimer, included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/ACE2 interaction.
The assay requires only a few steps. First, SARS-CoV-2 Spike Trimer (B.1.1.529 BA.1, Omicron Variant) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of chemiluminescence HRP substrate to produce the luminescence signal.
- PBS (Phosphate buffered saline)
- Rotating or rocker platform
- Luminescence microplate reader
| Catalog # | Name | Amount | Storage |
| 101343 | Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant), His-Tag (SARS-CoV-2)* | 20 µg | -80°C |
| 100665 | ACE2, His-Avi-Tag, Biotin-labeled HiP™* | 2 x 5 µg | -80°C |
| 79311 | 3x Immuno Buffer 1 | 50 ml | -20°C |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C |
| 79742 | Streptavidin-HRP | 10 µl | +4°C |
| 79670 | ELISA ECL Substrate A (translucent bottle) | 6 ml | Room Temp |
| ELISA ECL Substrate B (brown bottle) | 6 ml | Room Temp | |
| 79699 | White 96-well microplate | 1 | Room Temp |
*The initial concentration of both ACE2 and Spike Trimer is lot-specific and will be indicated on the tube containing the protein.
The COVID-19 pandemic is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit contains the receptor binding domain (RBD) which recognizes and attaches to the ACE2 receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. SARS-CoV-2 Variant B.1.1.529 BA.1, also known as Omicron variant, was originally discovered in South Africa and has recently become a global variant of concern. This variant contains a number of mutations that increase infectivity and transmissibility.
Drugs targeting the interaction between SARS-CoV-2 Spike protein and human ACE2 may offer some protection against viral infection. This kit includes the SARS-CoV-2 Spike Trimer (B.1.1.529 BA.1, Omicron Variant) protein in its native trimeric conformation to provide a relevant screen for inhibitors of the Spike S1:ACE2 interaction.
Hoffman M. et al., SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell 2020; 181:1-10.
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