Spike Trimer (S1+S2) (XBB.1, Omicron Variant), His-Tag (SARS-CoV-2) Recombinant

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101661

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Description

Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits. This protein corresponds to SARS-CoV-2 Omicron Variant XBB.1 and contains the Omicron Spike mutations listed below. The construct also contains a C-terminal His-tag. The recombinant protein was affinity purified.

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Product Data Gallery

Coomassie Staining

Binding Assay

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Mutation

T19I, LPP24-26del, A27S, V83A, G142D, Y144del/H146Q, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

1X PBS, 0.025% NaN₃

Apparent MW of >175 kDa in SDS-PAGE due to glycosylation

Tag(s)

C-terminal His-tag

Storage/Stability

At least 6 months at -80°C. This protein is highly sensitive to freeze/thaw cycles.

Assay Conditions

The ELISA was performed by coating 100 µl of ACE2-Fc at 2 µg/ml in PBS at 4˚C overnight. The plate was washed in PBS and blocked with 200 µl of 1% BSA in PBS for 1 hour at room temperature. Increasing amounts of Spike Trimer XBB.1 were added to the plate (serially diluted in blocking buffer). The plate was washed in PBS before adding 100 µl of AffiniPure Peroxidase-conjugated Anti-His-Tag antibody (Jackson Immuno Research #300-035-240) diluted 1:5,000 in blocking buffer for 1 hour at room temperature. The plate was washed with PBS and 100 µl of MB/E Ultra-Sensitiv HRP Substrate (EMD Millipore #ES022-500ml) was added for 5 minutes at room temperature. The reaction was stopped with 100 µl of 0.6N H₂SO₄ and read at 450 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.

Applications

Screen or titrate anti-spike antibodies or inhibitors; use as antigen control in assay design and optimization.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Scientific Category

Coronavirus

Regulatory Status

Research Use Only