SARS-CoV-2 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Colorimetric Assay Kit
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit has a receptor binding domain (RBD) which recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection. The SARS-CoV-2 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Assay Kit includes the Spike protein in its native trimeric conformation to provide a more physiologically relevant screen for inhibitors of the Spike S1:ACE2 interaction.
The SARS-CoV-2 Spike Trimer (S1+S2):ACE2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is that the SARS-CoV-2 Spike Trimer (S1+S2) protein provides a more biologically relevant model than monomeric Spike RBD protein for the investigation of SARS-CoV-2/host cell interaction. Only a few simple steps on a microtiter plate are required for the assay. First, SARS-CoV-2 Spike Trimer (S1+S2) is coated on a 96-well plate. Next, Biotin-ACE2 is incubated with SARS-CoV-2 Spike Trimer (S1+S2) on the plate. Finally, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which then can be quenched and measured using a UV/Vis microplate reader.
PBS (Phosphate buffered saline)
PBST (Phosphate buffered saline containing 0.05% Tween-20)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
| Catalog Number |
Component |
Amount |
Storage |
|
| 100728 | Spike Trimer (S1 + S2), His-Tag (SARS-CoV-2)* | 5 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 100665 | ACE2, His-Avi-Tag, Biotin labeled* | 5 µg | -80°C | |
| 79311 | 3x Immuno Buffer 1 | 50 ml | -20°C | |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C | |
| 79742 | Streptavidin-HRP | 10 µl | +4°C | |
| 79651 | Colorimetric HRP substrate | 10 ml | +4°C | |
| 79964 | Transparent 96-well microplate | 1 | Room Temp |
|
*The initial concentration of Spike Trimer and ACE2 is lot-specific and will be indicated on the vials containing the proteins.
Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. 2020. Science 367 (6485): 1444-1448.
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