Spike Trimer (S1+S2), His-tag, Eu-labeled (SARS-CoV-2) Recombinant

Catalog #

100894

$295 *

Size: 25 µg

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Description

Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein contains mutations 682RRAR685>A, K986P and V987P. The construct also contains a C-terminal His tag (6xHis). The recombinant protein was affinity purified and labeled with trFluor-Eu.

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Synonyms

Severe acute respiratory Coronavirus Spike trimer, SARS Cov-2 mutant, S protein, Spike S1, Spike S2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

SARS-CoV-2 Spike Trimer (Eu-labeled)

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Construct

Spike Trimer (S1+S2)(SARS-CoV-2) (16-1213-His)-( trFluor-Eu)

Host Species/Expression System

HEK293

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole and 20% glycerol.

139 kDa + glycans

Amino Acids

16-1213

Glycosylation

This protein runs at higher M.W. by SDS-PAGE due to glycosylation.

Genbank #

MN908947

Tag(s)

C-terminal His-tag

Label

trFluor™ Eu (Ex/Em = 346nm / 617 nm) is a fluorophore with large Stokes shifts and an extremely long emission half-live. It is ideal for applications that require high sensitivity and for time-resolved fluorometry because a delay between excitation and the detection of emission minimizes interference. EU-labeled probes are characterized by a relatively high stability and are often insensitive to quenching. trFluor™ Eu is covalently and randomly conjugated to the target protein.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

SARS-CoV2-Trimer Eu-labeled protein (6.25 nM) was incubated with ACE2, His-Avi-Tag, Biotin-labeled HiP (BPS Bioscience, #100665) and the Dye labeled acceptor in ACE2-Spike TR-FRET buffer for 1 hour at room temperature. TRFRET signals were measured and the ratio was calculated as [Ex340nm/Em665nm] /[Ex340nm/Em620nm].

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike Trimer (S1+S2), His-tag, Eu-labeled (SARS-CoV-2) Recombinant

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