Spike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit
The Spike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between SARS-CoV-2 Spike and human ACE2. The SARS-CoV-2 Spike Trimer, included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/host cell interaction.
The assay requires only a few steps. First, SARS-CoV-2 Spike Trimer is coated on a 96-well plate overnight. After blocking, the protein is pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.
PBS (Phosphate buffered saline)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm
| Catalog # | Name | Amount | Storage |
| 101165 | Spike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant), His-Tag (SARS-CoV-2) * | 5 µg | -80°C |
| 100665 | ACE2, His-Avi-Tag, Biotin-Labeled * | 2 x 5 µg | -80°C |
| 79311 | 3x Immuno Buffer 1 | 50 ml | -20°C |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C |
| 79742 | Streptavidin-HRP | 10 µl | +4°C |
| 79651 | Colorimetric HRP substrate | 10 ml | +4°C |
| 79964 | Transparent 96-well microplate | 1 | Room Temp |
*The initial concentration of both ACE2 and Spike Trimer is lot-specific and will be indicated on the tube containing the protein.
The COVID-19 pandemic is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit contains the receptor binding domain (RBD) which recognizes and attaches to the ACE2 receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. SARS-CoV-2 Variant B.1.617.2.1, also known as variant Delta Plus, was originally discovered in India and contains deletion Δ156/157 and mutations T19R, G142D, R158G, K417N, L452R, T478K, D614G, P681R, D950N.
Drugs targeting the interaction between SARS-CoV-2 Spike protein of and human ACE2 may offer some protection against viral infection. This kit includes the Spike Trimer (S1+S2) (B.1.617.2.1; Delta Plus Variant) (SARS-CoV-2) (BPS Bioscience #101165) in its native trimeric conformation to provide a physiologically relevant screen for inhibitors of the Spike S1:ACE2 interaction.
Hoffman M. et al., SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell 2020; 181:1-10.
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