Spike (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter)

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78348

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Description

The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of the viral replication, the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer protection against the viral infection. A variant called B.1.1.529 BA.1 (also known as the Omicron Variant) was identified in South Africa in November of 2021. This variant has a large number of mutations that allow the virus to spread more easily and quickly than other variants.

The Spike (B.1.1.529 Variant) (SARS-CoV-2) Pseudotyped Lentivirus were produced with SARS-CoV-2 B.1.1.529 BA.1 Variant Spike (Genbank Accession #QHD43416.1 with B.1.1.529 BA.1 mutations; see below for details) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions contain the firefly luciferase gene driven by a CMV promoter (Figure 1), therefore, the spike-mediated cell entry can be measured via luciferase activity. The Spike (B.1.1.529 Variant) (SARS-CoV-2) pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 B.1.1.529 BA.1 variant in a Biosafety Level 2 facility.

As shown in Figures 2 and 3, the Spike Omicron pseudovirus has been validated for use with target cells ACE2-HEK293 (which overexpress ACE2; BPS Bioscience, #79951).

Spike Mutations in B.1.1.529 BA.1 Variant:

A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F

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Synonyms

south africa variant, south africa lenti, luciferase reporter, sublineage BA.1

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Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Replication Incompetent HIV

Supplied As

The titer will vary with each lot; the exact value is provided with each shipment. Based on experiments performed by scientists at BPS Bioscience, 79942-1 (100 µl) provides sufficient signal-to-noise ratio to perform 100 reactions, and 79942-2 (500 µl x2) for 1000 reactions. The amount of virus added to the cells can even be titrated down according to the user’s need.

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 1 or HEK293 Growth Medium	BPS Bioscience #60187
ACE2-HEK293 Recombinant Cell Line	BPS Bioscience #79951
Spike Neutralizing Antibody (Clone C-A11) (SARS-CoV-2)	BPS Bioscience #101024
Spike Neutralizing Antibody (Clone G10xA1) (SARS-CoV-2)	BPS Bioscience #101326
Spike Neutralizing Antibody (Clone G10xA5) (SARS-CoV-2)	BPS Bioscience #101327
96-well tissue culture treated, white clear-bottom assay plate	Corning #3610
ONE-Step™ luciferase assay system	BPS Bioscience #60690

Formulation

The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS.

Media Formulation
Thaw Medium 1 (BPS Bioscience #60187):
MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Applications

Screen for or titrate neutralizing antibodies against SARS-CoV-2 Spike in ACE2-HEK293 cells

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Biosafety
None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter)

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