Spike (SARS-CoV-2) Lentivirus

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Catalog #

78010

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Description

Cell entry of SARS-CoV-2 depends on the binding of viral spike protein to cellular receptor ACE2. The SARS-CoV-2 Spike Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. The particles contain the full length SARS-CoV-2 spike gene (QHD43416.1) driven by an EF1A promoter (Figure 1).

78010

Figure 1. Schematic of the lenti-vector used to generate the SARS-CoV-2 spike lentivirus Storage

This product has been cited 15 times.

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Synonyms

Coronavirus Spike, pseudovirus, pseudovirion,

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Product Info

Storage and Usage

Citations15

Supplied As

The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Genbank #

QHD43416.1

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions

Shipping Temperature

-80°C

Warnings

Biosafety
None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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Spike (SARS-CoV-2) Lentivirus

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