PD-1:PD-L1 TR-FRET Assay
The PD-1:PD-L1 TR-FRET Assay is designed to measure the inhibition of PD-1 binding to PD-L1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L1, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
Catalog # | Component | Amount | Storage | |
PD-1-Eu | 8 µg | -80°C |
Avoid |
|
71105 | PD-L1, Biotinylated | 30 µg | -80°C | |
Dye-labeled acceptor | 2 x 10 µl | -20°C | ||
79311 | 3x Immuno Buffer 1 | 4 ml | -20°C | |
White, non-binding, low volume, 384-well microtiter plate | 1 | Room Temp. |
1. Molnar, E., et al. Proc Natl Acad Sci USA. 2008; 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008; 26: 677-704.