PD-1, FLAG-Avi-His-Tag, Biotin-Labeled (Human) HiP™ Recombinant

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Catalog #
71325
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Description

Recombinant human PD-1 (programmed cell death 1), encompassing amino acids 25-167. The construct contains a C-terminal FLAG-tag followed by an Avi-Tag™ and a His-tag (10xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.

Synonyms
PDCD1, programmed cell death 1, PD1, hPD-1, hPD-l, CD279, SLEB2, hSLE1
Product Info
Storage and Usage
Citations
Species
Human
Construct
PD-1 (25-167-FLAG-Avi-His)-(Biotin)
Host Species/Expression System
HEK293
Purity

≥90%

Format
Aqueous buffer solution
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
MW
20 kDa + glycans
Amino Acids
25-167
Glycosylation
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Aggregation
<10%
Genbank #
NM_005018
UniProt #
Q15116
Tag(s)
C-terminal FLAG-Avi-His-tags
Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note

References
1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.
2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.