PD-1:PD-L2 TR-FRET Assay Kit

Catalog #
72012
$1,070 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The PD-1:PD-L2 TR-FRET Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2 in a homogeneous 384 reaction format. Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L2, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

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This product has been cited 1 time.

Product Info
Storage and Usage
Citations1
Assay Kit Format
TR-FRET
Supplied As
This kit comes in a convenient 384-well format, with Europium-labeled PD-1 and biotin-labeled PD-L2.
Materials Required But Not Supplied
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET).
Adjustable micropipettor and sterile tips.
Format
Catalog Number
Component

Amount

Storage
PD-1-Eu (Human) 2 µg -80°C




(Avoid
f
reeze/thaw cycles!)

71108 PD-L2, Biotinylated (Human) 20 µg -80°C
Dye-labeled acceptor 2 x 10 µl -20°C
79311 3x Immuno Buffer 1 4 ml -20°C
White, non-binding, low volume, 384- well microtiter plate 1 Room temp.
UniProt #
PD-1: Q15116; PD-L2: Q9BQ51
Background
Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses.
References

1. Molnar, E., et al. Proc Natl Acad Sci USA. 2008; 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008; 26: 677-704.