PD-1 (Mouse) / NFAT - Reporter - Jurkat Recombinant Cell Line

Catalog #
79762
$11,095 *
Size: 2 vials
Qty
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Description

Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of mouse PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_008798).

Interested in screening and profiling inhibitors, blocking antibodies, or activators of mouse PD-1 without the need to purchase and license the cell line? Check out our Immunotherapy Cell-Based Screening Services.

This product has been cited 3 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
Programmed Cell Death 1, PDCD1, SLEB2, CD279
Product Info
Storage and Usage
Citations3
Host Cell Line
Jurkat T Cell
Species
Mouse
Supplied As
Each vial contains 2 x 10^6 cells in 1 ml of 90% FBS, 10% DMSO
Materials Required But Not Supplied

Mouse PD-L1/TCR activator-CHO cell line (BPS Bioscience #79763)
Assay medium: Thaw Medium 2 (BPS Bioscience #60184)
Growth Medium 2F (BPS Bioscience #79669)
• Anti-mouse PD-1 neutralizing antibody: Bioxcell #BP0273, clone#29F.1A12
• Anti- PD-L1 neutralizing antibody (Fisher Scientific #50-146-65, clone#MIH5)
• 96-well tissue culture-treated white clear-bottom assay plate
One-Step luciferase assay system (BPS Bioscience #60690) or other luciferase
reagents for measuring firefly luciferase activity
• Luminometer
Thaw Medium 3 (BPS Bioscience #60186): Ham’s F-12 medium (Hyclone
#SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1%
Penicillin/Streptomycin (Hyclone #SV30010.01).

UniProt #
Q02242
Mycoplasma Testing

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

Background

The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

Assay Principle