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Description
The PD-1:PD-L2 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated with the biotinylated PD-L2 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Need us to run inhibitor screens or profile your compounds against PD-1:PD-L2? Check out our Immunotherapy Biochemical Screening Services.
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Synonyms
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC
Product Info
Storage and Usage
Citations
Supplied As
The PD-1:PD-L2 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated PD-L2, FLAG-tagged PD-1, and assay buffer to perform a total of 384 reactions.
UniProt #
PD-1: Q15116; PD-L2: Q9BQ51
References
1. Molnar, E. et al. Proc. Natl. Acad. Sci. U.S.A. 2008; 105: 10483-10488
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008; 26: 677-704.
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol
Applications
Useful for screening for inhibitors of PD-1 binding to PD-L2
Shipping Temperature
-80°C (dry ice)
Notes
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Contraindications
Only limited amounts of DMSO can be included, as it has been shown
to disrupt PD-1:PD-L2 interaction. Avoid green and blue dyes that absorb light in the
AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the
potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+,
Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due
to the presence of excess biotin and iron in this medium. MEM, which lacks these components,
does not affect AlphaScreen assays.
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to
[email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
Scientific Category
Immunotherapy
Regulatory Status
Research Use Only
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