Monkey PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit

Catalog #
79863
$820 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and this pathway is exploited by both tumors and viruses. The Monkey PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling pathway in the animal model cynomolgus monkey (Macaca fascicularis).

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Synonyms
Programmed Cell Death 1 Ligand 1 assay kit, CD274 assay kit, B7 homolog 1 (B7-H1) assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
This kit comes in a convenient 96-well format, with biotin-labeled monkey PD-L1, purified monkey PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled PD-L1
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format
Catalog # Component  Amount Storage
71158 PD-1 (Monkey), Fc fusion (Human IgG1) HiP™ 10 µg -80°C  Avoid
freeze/
thaw
cycles!
71154 PD-L1 (Monkey), Fc fusion (Human IgG1),  Biotin-labeled HiP™   5 µg -80°C 
79311 3x Immuno Buffer 1 50 ml  -20°C 
79728  Blocking Buffer 2 50 ml  +4°C 
79742 Streptavidin-HRP  10 µl  +4°C
79670 ELISA ECL Substrate A 6 ml Room Temp
ELISA ECL Substrate B 6 ml Room Temp
79699 96-well white microplate  +4°C
 
 
 
 
 
                                                                               
 
References

1. Onlamoon, N. et al. Immunol. 2008, 124.1: 277-293
2. Wang. C. et al. Cancer Immunol. Res. 2014, 2.9: 846-856