PD-L1 (CD274), Fc fusion (Human) HiP™ Recombinant

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Catalog #

71104

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Description

Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 52 kDa.

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Synonyms

Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PD-L1: PD-1[Biotinylated] Binding

Gel Filtration Curve

PD-L1:B7-1 [Biotinylated] inhibition curve using a colorimetric assay (#72026). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

52 kDa

Amino Acids

19–239

Genbank #

NM_014143

UniProt #

Q9NZQ7

Tag(s)

C-terminal Fc fusion (Human IgG1)

Background

PD-L1 is involved in the costimulatory signal essential for T-cell proliferation and production of IL-10 and IFNgamma. Interaction with PD-1 inhibits T-cell proliferation and cytokine production, and down-regulates the immune response.

References

1. Freeman, G.J., et al., J Exp Med. 2000 Oct 2;192(7):1027-34.
2. Dong, H., et al., Nat Med. 1999 Dec; 5(12):1365-9.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

PD-L1 binding to PD-1 was tested following protocol for BPS Cat. #72005. Briefly, wells were coated with 10 ng of PD-L1 in PD-1 assay buffer. Varying concentrations of biotinylated PD-1 was added and wells were incubated at room temp. for 2 hours before adding strep-HRP, followed by chemiluminescent substrate. Luminescence was read on a chemiluminescent plate reader.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71104-1

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PD-L1 (CD274), Fc fusion (Human) HiP™ Recombinant

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