PD-L1:B7-1 [Biotinylated] Inhibitor Screening Assay Kit (Mouse)

Catalog #

79821

$790 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PD-L1:B7-1 [Biotinylated] Inhibitor Screening Assay Kit (Mouse) is designed for screening and profiling inhibitors of murine PD-L1 binding to murine B7-1. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, biotinylated B7-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against PD-L1:B7-1 [Biotinylated] (Mouse)? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

CD274 assay kit, PD-L1 assay kit, B7H1 assay kit, B7 assay kit, CD80 assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format

Catalog #	Component	Amount	Storage
71117	PD-L1 (CD274), Fc fusion (Mouse) HiP™	30 µg	-80°C	Avoid multiple freeze/ thaw cycles!
100478	B7-1 (CD80) (Mouse), Fc fusion, Avi-Tag, Biotin-labeled, HiP™	30 µg	-80°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79742	Streptavidin-HRP	10 µl	-20°C
79670	ELISA ECL Substrate A	6 ml	Room Temp
79670	ELISA ECL Substrate B	6 ml	Room Temp
79699	96-well white microplate	1	+4°C

UniProt #

PD-L1: Q9EP73; B7-1: Q00609

Background

B7-1, also known as CD80, is a membrane receptor found in activated antigen presenting cells (APC). In addition to PD-1, PD-L1 is able to bind to B7-1, resulting in inhibition of T cell activation and cytokine production. This immune checkpoint is another target of interest for immunoregulatory functions in oncology and other disease areas. Mouse PD-L1 and B7-1 are the respective orthologs of the human proteins, so they can be used for research in animal models.

References

Butte, M. J., et al. mmunity 2007, 27(1):111-22
Chaudhri, A., et al. Cancer Immunol Res 2018, 6(8): 921-929

Storage/Stability

Up to 6 months from date of receipt, when stored as recommended.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze / thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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PD-L1:B7-1 [Biotinylated] Inhibitor Screening Assay Kit (Mouse)

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