PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

72026

$1,215 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of the PD-L1:B7-1 interaction. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, B7-1-biotin is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against PD-L1:B7-1 [Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1), CD80, CTLA-4 Counter-Receptor B7.1 , activity assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled B7-1 (CD80), purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Format

Catalog Number	Component	Amount	Storage
71104	PD-L1, Fc fusion	25 µg	-80°C	Avoid freeze/ thaw cycles!
71114	B7-1 (CD80), Fc fusion, Biotin-labeled	15 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp.
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp.
79699	White 96-well microplate	1	+4°C

UniProt #

PD-L1: Q9NZQ7; B7-1: P33681

Background

There have been a number of studies defining a role for PD-L1 in the regulation of immune tolerance and cancer immunity through binding to T cell-expressed PD-1. Interestingly, PD-L1 has also been shown to interact with B7-1, also known as CD80. Like PD- 1:PD-L1 interaction, B7-1:PD-L1 signaling also leads to inhibition of T cell activation, proliferation, and cytokine production, suggesting that this pathway may also serve as a target in cancer immunotherapy and inflammatory diseases.

References

1. Butte, M.J., et al., Immunity. 2007; 27(1): 111-122.
2. Butte, M.J. et al., Mol. Immunol. 2008; 45: 3567-3572.

Storage/Stability

Stable for 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

This kit is useful for screening for inhibitors of PD-L1 binding to B7-1.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only