PD-L1 (Mouse)/TCR Activator CHO Cell Line
PD-L1 (Mouse)/TCR Activator CHO Cell Line is a CHO-K1 cell line constitutively expressing mouse PD-L1 (Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7- H1), GenBank accession #NM_021893) and an engineered T cell receptor (TCR) activator. Surface expression of PD-L1 was confirmed by flow cytometry, and the cell line was functionally validated in a mouse PD-1:PD-L1 cell-based assay.
Figure 1: Mechanism of action of PD-L1 (Mouse)/TCR Activator CHO Cell Line in combination with the Mouse PD-1/NFAT reporter-Jurkat cell line. Jurkat T cells expressing NFAT reporter with constitutive expression of mouse PD-1 (mouse PD-1/NFAT Reporter Jurkat Cell Line, BPS Bioscience #79762) act as effector cells. When co-cultivated, TCR complexes on mouse PD-1/NFAT Reporter Jurkat cells are activated by the TCR activator of PD-L1 (Mouse)/TCR Activator CHO Cell Line, resulting in expression of the NFAT luciferase reporter. However, PD-1 and PD-L1 ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-mouse PD-1 or anti-mouse PD-L1 antibodies. Mouse PD-1/PD-L1 neutralizing antibodies block PD-1:PD-L1 interaction and promote T cell activation, resulting in reactivation of the NFAT responsive luciferase reporter.
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Media Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 3 | BPS Bioscience #60186 |
| Growth Medium 3A | BPS Bioscience #60188 |
Materials Required for Cellular Assays
| Name | Ordering Information |
| Thaw Medium 2 | BPS Bioscience #60184 |
| Mouse PD-1/NFAT Reporter-Jurkat Cell Line | BPS Bioscience #79762 |
| NFAT Luciferase Reporter Jurkat Cell Line | BPS Bioscience #60621 |
| Anti-mouse PD-1 Neutralizing Antibody | Bioxcell #BP0273 |
| Anti-mouse PD-L1 Neutralizing Antibody | Fisher Scientific #50-146-65 |
| 96-well tissue culture-treated white clear-bottom assay plate | |
| One-Step™ Luciferase Assay System | BPS Bioscience #60690 |
| Luminometer |
The cell line has been screened to confirm the absence of Mycoplasma species.
The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. PD-1 ligands are found in most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.
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