B7-1(CD80) (Mouse), Fc fusion, Avi-Tag, Biotin-labeled, HiP™ Recombinant

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Catalog #

100478

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Description

Mouse secreted B7-1, also known as CD80, GenBank Accession No. NM_009855, a.a 38-246, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotinylated using Avi-tag™ technology. MW=52 kDa. Biotinylation is confirmed to be ≥90%.

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Synonyms

CTLA-4 Counter-Receptor B7.1, CD80

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Mouse CTLA4: B7-1 [Biotin] Interaction

Gel Filtration Curve

PD-L1:B7-1 [Biotinylated] (Mouse) inhibition curve using a chemiluminescent assay (#79821). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

HEK293

Purity

≥90%

Supplied As

Aqueous buffer solution.

52 kDa

Amino Acids

38-246

Genbank #

NM_009855

UniProt #

Q00609

Tag(s)

C-terminal Fc-Avi-tag

References

1. Pentcheva-Hoang, T., et. al. Immunity. 2004 Sep; 21(3): 401-413.
2. Hirano, F., et al., Cancer Research. 2005 Feb; 65(3): 1089-1096.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Reaction done similar to CTLA4:B7-1[biotin] assay kit (Catalog # 72009) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20.
Coat protein (mouse CTLA4) was added overnight at 4 deg 100 ng/well. Various amounts of biotinylated ligand (mouse B7-1[B]) (0-50 ng) added for 2h at RT. Strep-HRP added for 1h at RT.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

100478 Lot 190815.pdf

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B7-1(CD80) (Mouse), Fc fusion, Avi-Tag, Biotin-labeled, HiP™ Recombinant

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