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Description
Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD- 1[Biotinylated]:PD-L2 Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-1 receptor binding to PD-L2 ligand. The key to this kit is the high affinity of biotin-labeled PD-1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
Need us to run inhibitor screens or profile your compounds against PD-1[Biotinylated]:PD-L2? Check out our Immunotherapy Biochemical Screening Services.
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Synonyms
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC, activity assay kit
Product Info
Storage and Usage
Citations
Supplied As
This kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L2, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
UniProt #
PD-1: Q15116; PD-L2: Q9BQ51
References
1. Lazar-Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol
Applications
This kit is most useful for screening for inhibitors of PD-L2.
Shipping Temperature
-80°C (dry ice)
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to
[email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
Scientific Category
Immunotherapy & Cell Surface Receptors
Regulatory Status
Research Use Only