PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit

Catalog #
72006
$1,235 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-L2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

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Synonyms
activity assay kit, Strep HRP, streptavidin
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Species
Human
Supplied As
This kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L2, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Format
Catalog
Number

Component

Amount

Storage
71109 PD-1, Biotin-labeled 2 µg -80°C






Avoid
freeze/
thaw
cycles!
71107 PD-L2 10 µg -80°C
79742 Streptavidin-HRP 10 µl +4°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C
79670 ELISA ECL substrate A
(transparent bottle)
6 ml Room
Temp
ELISA ECL substrate B
(brown bottle)
6 ml Room
Temp
79699 White 96-well microplate 1 +4°C

UniProt #
PD-1: Q15116; PD-L2: Q9BQ51
References
1. Lazar-Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.