PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit
Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-L2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
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PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform
Catalog Number |
Component |
Amount |
Storage |
|
71109 | PD-1, Biotin-labeled | 2 µg | -80°C | Avoid freeze/ thaw cycles! |
71107 | PD-L2 | 10 µg | -80°C | |
79742 | Streptavidin-HRP | 10 µl | +4°C | |
79311 | 3x Immuno Buffer 1 | 50 ml | -20°C | |
79728 | Blocking Buffer 2 | 50 ml | +4°C | |
79670 | ELISA ECL substrate A (transparent bottle) |
6 ml | Room Temp |
|
ELISA ECL substrate B (brown bottle) |
6 ml | Room Temp |
||
79699 | White 96-well microplate | 1 | +4°C |
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.