PD-1: PD-L1/L2 & PD-1:B7-1 Screening Services

BPS provides PD-1 biochemical and cell-based screening and profiling services. Biochemical screening services provide a great initial screen to find small molecules or biologics that prevent PD-1 from binding to its ligands; PD-L1, PD-L2, and B7-1. Then, the most promising hits can be further screened in our PD-1 reporter cell-based assay which offers a functional readout of the result (agonistic vs antagonistic) on PD-1 signaling. Trust us to provide you with high quality data with fast turnaround time. Our team of experts along with our broad services portfolio makes it easy to:

  • Screen for inhibitors/targets using our PD-1 assays
  • Select from IC50 determination and single point concentrations
  • Receive data within days of compound submission
  • Perform follow-up studies using the same soluble receptors manufactured in-house
  • Get questions answered or project guidance in a time-efficient manner

Contact BPS today to find out more about how we can assist you in your PD-1 related research.

Biochemical Assay Format:

Cell-Based Assay Format: 

Jurkat T cells stably expressing human PD-1 and NFAT responsive luciferase reporter are used as effector cells; CHO cells over-expressing PD-L1 or PD-L2 and an engineered TCR activator are used as target cells. When these two cells are co-cultivated, TCR complexes on effector cells are activated by TCR activator in target cells, resulting in expression of NFAT luciferase reporter. However, PD-1:PD-L1/PD-L2 ligation prevents TCR activation and suppresses the NFAT responsive luciferase activity. This inhibition can be specifically reversed by anti-PD1 Ab (Fig 1a) or anti-PD-L1 Ab (Fig 1b). PD-1 or PD-L1 neutralizing Ab blocks PD-1:PD-L1 interaction and therefore promotes T cell activation, resulting in re-activated NFAT responsive luciferase reporter.

Fig 1a:

Fig 1b:

This reporter based PD-1 cell based assay provides a tool for PD-1 drug screening and characterization that complements BPS' PD-1 biochemical screens.

We also offer custom assays for several other immunotherapy targets. Our Custom Assay Development Services allow for flexibility to meet your specific research needs.

BPS Bioscience offers many different types of PD-1 assay kits for screening inhibitors of PD-1:PD-L1 or PD-L2 interaction: