PD-L1 (CD274), Fc fusion, Biotin-labeled (Human) HiP™ Recombinant

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Catalog #

71105

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Description

Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, encompassing amino acids 19-239, fused at the C-terminus with the Fc portion of human IgG1 followed by an Avi-tag™. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration

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Synonyms

Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PD-1:PD-L1[Biotinylated] Binding

Biotin-Avidin Pulldown

Gel Filtration Curve

PD-1: PD-L1 inhibition curve using a TR-FRET assay (#72032). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

PD-L1 (19-239-Fc(IgG1)-Avi)-(Biotin)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

54 kDa + glycans

Amino Acids

19–239

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Aggregation

<10%

Genbank #

NM_014143

UniProt #

Q9NZQ7

Tag(s)

C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Background

Programmed Cell Death 1 Ligand 1 (PD-L1), also known as CD274 and B7 homolog 1 (B7-H1), is an immune checkpoint acting as a negative regulator of immune cells. It helps maintain immune homeostasis and prevent excessive immune responses. Normally expressed at the surface of antigen-presenting cells, it is also found in tumor cells and contributes to cancer-associated immune suppression through its interaction with PD-1 expressed in T cells. Checkpoint inhibitors targeting the interaction between PD-L1 and PD-1 (such as pembrolizumab and nivolumab) have demonstrated significant clinical activity in various types of cancer and proved transformative for cancer therapy.

References

1. Freeman, G.J., et al., J Exp Med. 2000 Oct 2;192(7):1027-34.
2. Dong, H., et al., Nat Med. 1999 Dec;5(12):1365-9.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit (BPS Bioscience #72003) with PD-L1 (CD274), Fc Fusion, Biotin-labeled (Human) Recombinant titrated at various concentrations

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for binding studies and screening inhibitors of PD1 binding to PD-L1.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71105 Lot 230801-G

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PD-L1 (CD274), Fc fusion, Biotin-labeled (Human) HiP™ Recombinant

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